Figure 5.

RSK4 could promote the invasive and metastatic activities of RCC cells. (A) RSK4-overexpressing ACHN cells showed elevated numbers of invasive and metastatic cells compared with normal and vector control cells (above, *P<0.05 vs normal and vector). When RSK4 was downregulated by shRNA in GRC-1 cells, the number of invasive and metastatic cells were reduced (below, *P<0.05 vs normal and scrambled). (B) RSK4-overexpressed ACHN cells showed increased level of CD44 and MMP-9; however, RSK4-suppressed GRC-1 cells manifested with decreased level of CD44 and MMP-9. β-Actin was showed as loading control (RSK4 antibody for immunoblot: JS-31, Santa Cruz Biotechnology).