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Loading of NGF into collagen microspheres. (A) Confocal images of loaded spheres (green, collagen spheres; red, NGF; yellow, colocalization of NGF and collagen). (B) Loading efficiency with different doses of NGF (LH panel) and into three types of spheres (0× non-cross-linked, 1× cross-linked, and 2× highly cross-linked (RH panel). Data represents mean ± SD, n = 3.
