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EMSA of ΔN20C17-HMGA1b with A19-DNA (A, B), and A5T5-DNA (C). Amino acid sequence of HMGA1a and HMGA1b deletion mutants (D). The AT-hook sequences are underlined and labeled. In ΔN20C17-HMGA1a, the insert sequence is indicated, and in ΔN20C17-HMGA1b, the deletion of the insertion region (boxed) results in a lysine residue (arrow) being to close to first AT-hook (underlined). The molar ratio of ΔN20C17-HMGA1b to DNA in each lane is indicated below the gel on the left.
