Skip to main content
. 2013 Sep 13;455(Pt 1):107–118. doi: 10.1042/BJ20130126

Figure 4. Activity of EndoS2 on IgG, IgG subclasses and AGP.

Figure 4

(A) Human serum IgG was incubated with recombinant EndoS2, EndoS2(E186L), EndoS, PNGase F or PBS at 37°C in PBS overnight and analysed by SDS/PAGE (10% gel) and a subsequent LCA blot. The gel and lectin blot shows the γ heavy chain of IgG at 50 kDa. (B) Human subclasses of IgG, IgG1–4, were incubated with recombinant EndoS2, EndoS2(E186L) or PBS in PBS at 37°C overnight and analysed by SDS/PAGE (10% gel) and a subsequent LCA lectin blot. (C) AGP was incubated with recombinant EndoS2, EndoS or PBS at 37°C in PBS overnight and analysed by SDS/PAGE (10% gel).