Figure 2.

Knockdown of HAS2 partially inhibits TGFβ-induced EMT. NMuMG cells were transfected with scrambled siRNA or siRNA against HAS2 or CD44, starved and stimulated with 1 ng/ml TGFβ for up to 48 h. Phase contrast micrographs were taken with a Zeiss Axiovision 40 phase contrast microscope. Scale bar=100 μm. The number of epithelial cells (vs mesenchymal cells) was counted in about eight photos per treatment and the average ±s.d. from three separate experiments is shown to the right. Asterisks indicate statistically significant differences comparing TGFβ-stimulated cells transfected with siRNA against HAS2 with cells transfected with scrambled siRNA.