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. 2013 Sep 6;4:2406. doi: 10.1038/ncomms3406

Figure 2. Quantitative analysis of LPS stimulation.

Figure 2

Naive B cells were labelled with CFSE and stimulated with indicated concentrations of LPS. (a) Measurement of total cell numbers over time in response to LPS titration. Data points=mean±s.e.m. of triplicate cultures. (b) Division progression of B cells was determined by dilution of CFSE. (c) Colcemid plot of mean time to first division in response to LPS stimulation. Data points=mean±s.e.m. of triplicate cultures (d) Cplot1 of cohort analysis of LPS data to visualize the change in maximum division number (division destiny) and division rate with LPS concentration. Red line=intercept with 1 and approximate time to first division. (e) Cplot2 was used to approximate changes in death with concentration of LPS. Error bars in d and e represent 95% confidence intervals (f) Proportion of syndecan-1+ cells per division was measured at day 4 in cultures containing CFSE-labelled B cells stimulated with either LPS (left panel) or LPS and IL-4 (right panel). Data points in f represent the mean and s.e.m. of four replicate samples. All data are representative of four independent experiments.