Fig. 3.
Examples of dose responses of transfected HEK293 cells containing mouse ER (mERα), marisa ER-like (mcER) or marisa ERR (mcERR) exposed to various chemicals. A) Response of mERα to E2 (10−10 to 10−6 M) and BPA (10−10 to 10−5 M) and mcERR to BPA (10−10 to 10−4 M). B) Response of mERα, mcER and mcERR to genistein from 10−11 to 10−4 M. The inclusion of the construct with the LBD excised plus chemical (Mock) and mERα with solvent carrier (control) were used as negative controls. Error bars denote the standard deviation of two replicate samples per treatment. Concentrations above 10−6 M showed signs of toxicity and are therefore not included.