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. 2009 Oct 16;393(1):202–213. doi: 10.1016/j.jmb.2009.08.027

Fig. 3.

Fig. 3

Mutagenesis of the CSPG binding surface of DBL3X. (a) An electrostatic representation of the surface of the DBL3X domain showing the positively charged patch and sulfate-binding site. A bound sulfate ion is shown in stick representation. The side chains that contact the sulfate (K1324 and R1467) are labeled, as are the other side chains that contribute to the positive charge. (b) Plot of the saturated Biacore response obtained against protein concentration for mutants of the DBL3X domain.