Figure 6.
Blocking IL-17A inhibits serum amyloid A (SAA)–induced neutrophil recruitment. Mice were pretreated with anti–IL-17A or rat IgG2A (isotype) antibody (50 μg) intranasally 1 hour before saline or SAA (2 μg) administration. (A) Representative fluorescence-activated cell sorter plot of each treatment group 24 hours after SAA administration. (B) Total number of neutrophils in the bronchoalveolar lavage (BAL) and (C) lung of treated mice. Pooled data from two independent experiments. n = 5–8 mice per group. Gene expression of the neutrophilic mediators (D) CXCL1 and (E) CXCL2 in the lung 6 hours after SAA administration. n = 4–5 mice, *P < 0.05.