Figure 4.

Testosterone Modulation of AR Activity during MYC Activation

(A–I) WT, K14MycER (Myc:), K14MycER AR-Shah, and K14MycER AR-TFM mice were treated as indicated. (A) Skin sections were stained with H&E. (B and C) Skin sections were stained with the antibodies shown. (D) Quantitation of the SG differentiation compartment. (E) Quantitation of Ki67+ve sebocytes per SG. (F) qRT-PCR of Nucleolin (Nuc) mRNA levels relative to Gapdh. (G and H) Skin sections were stained with H&E. Quantitation of SG differentiation compartment size is shown. (I) Schematic summary of the effect of testosterone on K14MycER mice treated with high-dose 4OHT. See Figure 1N for stages in sebocytes differentiation. Increases in cell number are represented by +, ++, or +++, according to the strength of the effect. Reduction in cell number is represented by −.

Three to six mice were examined per condition. Error bars represent SEM. ^∗p < 0.05, ^∗∗p < 0.01, and ^∗∗∗p < 0.005. Scale bars, 40 μm. See also Figures S3 and S4.