Figure 5.

Effect of MYC and AR Activation on p53 Expression, DNA Double Strand Breaks, and Apoptosis

(A–L) WT and K14MycER (Myc:) mice were treated as indicated. (A) Skin sections were labeled with antibodies to γ-H2AX with DAPI nuclear counterstain. (B, H, and K) Skin sections were labeled with antibodies to p53 (arrowheads indicate positive nuclei). (C) Skin sections were labeled with antibodies to cleaved caspase 3. (D–F) qRT-PCR of mRNA levels relative to Gapdh. (G, I, and L) Quantitation of p53+ve nuclei per unit area of skin (HF, SG, and overlying IFE). (J) qRT-PCR of p53 mRNA levels relative to Gapdh.

Three to nine mice were examined per condition. Error bars represent SEM. ^∗p < 0.05, ^∗∗p < 0.01. Scale bars, 40 μm.