Figure 6.

Loss of p53 Function during MYC Activation

(A–K) Mice were treated as indicated. (A and J) Skin sections were stained with H&E. (B) Quantitation of SG differentiation compartment. (C and J) Skin sections were stained with the antibodies shown. Arrowhead in (J) indicates sebocyte differentiation within the IFE. (D–I) qRT-PCR of mRNA levels relative to Gapdh. (K) Schematic summary of effects of loss of p53 and combined loss of p53 and the AR. See Figure 1N for stages in sebocytes differentiation. Increases in cell number are represented by +, ++, or +++, according to the strength of the effect. Reduction in cell number is represented by −.

Scale bars, 40 μm. Error bars represent SEM. Three to five mice were examined per condition, except n = 1 for triple cross. NS, not significant. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.005. See also Figure S5.