Fig. 5.

A seven-state kinetic scheme describes the voltage and ATP dependence of ClC-2 common gating. a Seven-state diagram representing slow gating of ClC-2. In the absence of ATP (left half), the slow gate cycle consists only of one closed (C) and two open (O _min, O _v) states with voltage-dependent opening transitions to O _v and voltage-independent transitions to O _min. ATP binding causes transition into a cycle with altered rate constants that result in slower gating kinetics. b Predicted current responses to the voltage steps seen in Fig. 1. c Predicted ATP dependence for wild type channels (solid line) follow measured values (open squares). d Predicted voltage dependence of time constants of WT (continuous lines, either at 0 or 5 mM ATP internally) correspond to measured values (grey and black squares)