Fig. 1.

Ceramide and sphingoid base composition of CD macrophages is unaltered. The amounts of individual ceramide species were quantified in HC (n = 7) and CD (n = 8) macrophages by HPLC-MS. (A and B) Pie charts depicting the proportion of various ceramides in HC and CD macrophages respectively, expressed as a percentage of the total amount of ceramide detected. (C) The amounts of individual ceramide species (in pmol/mg protein) in unstimulated HC and CD macrophages. No significant differences were identified between HC and CD. (D) The amounts of sphingosine (Sph) and dihydrosphingosine (dhSph) in HC and CD macrophages. (E) Total amounts of ceramide in HC and CD macrophages. Results are expressed as mean + SEM.