Figure 3.

A and B, HIV DNA (A) and RNA (B) with normalization to cell frequency (in 10⁶ WBCs). For each site (blood, ileum, rectum), we used the HIV levels within each cell type (Figure 2) and the frequency of each cell type (% of live singlet CD45⁺ cells, as determined by flow cytometry) to calculated the HIV content within each cell type in 10⁶ WBCs. Samples that had undetectable HIV levels were assigned a value equal to the detection limit of the assay. The HIV levels in T_R7+, T_TM, and T_EM CD4⁺ T cells were added to yield an estimate of the total HIV level in CD4⁺ T cells (triangles), which was added to the level in non-CD4⁺ T leukocytes (circles) to give an estimate of the total HIV level in WBCs (squares). Data are shown on a log scale. Bars represent the medians. Abbreviations: HIV, human immunodeficiency virus; WBC, white blood cell.