Fig. 2.

Expression of Dll4, Notch1, Hey1, PTEN, p-AKT, pan-AKT, and VEGF proteins in GBM. Total protein was extracted from the operative specimens of GBM (n = 15) and control tissues (normal brain, n = 5). The expression of Dll4, Notch1, Hey1, PTEN, p-AKT, AKT, and VEGF was detected using specific antibodies. Actin was measured as a housekeeping protein to control the total protein loaded into each lane. (A) A representative Western blot of GBM (G1–G6) and control (C1–C3) samples. (B) Semiquantification of protein levels. The optical density (OD) of the bands on blots representing individual specific proteins and the housekeeping protein actin was measured by ImageJ software. The OD ratio of the target protein to actin was calculated, and data are presented as a percentage of the control. *P < .05 and **P < .01, compared with the control.