Fig. 5.

Cellular localization of immunoreactivity of Dll4 and cleaved Notch1 in GBM. (A) Negative control without the primary antibody for immunostaining did not show a signal. (B) Immunohistochemistry of laminin revealed the typical classic neovascularization pattern of branching vessels (red asterisks) in GBM samples. (C–F) Immunohistochemistry of Dll4 (C and D) and cleaved Notch1 (E and F) in GBM samples. The immunoreactivity of Dll4 and cleaved Notch1 was frequently detected in the endothelial cells of classically patterned vessels (red asterisks in C and E), in macrophage-like cells (arrowheads in D and F), and in some tumor cells (arrows in D, E, and F).