Table 1.

Primer sequences and annealing temperatures for real-time reverse-transcription PCR

Primer	Sequence	Annealing temperature (°C)
DLL4
for.	GCG GGG TAC CTT CTC GCTCAT CAT C	60
rev.	GCC TCC CCA GCC CTC ATC ACA AGT A
Jagged1
for.	TCG CTG TAT CTG TCC ACC TG	60
rev.	AGT CAC TGG CAC GGT TGT AG
Notch1
for.	CAG GCA ATC CGA GGA CTA TG	60
rev.	CAG GCG TGT TGT TCT CAC AG
Notch4
for.	TCC TGG GGC CCG GGC TGA AGA AAA G	58
rev.	ACG CCG GAT GAG CTG GAG GAC GAG A
HEY1
for.	CAGGCAATCCGAGGACTATG	60
rev.	CAGGCAATCCGAGGACTATG
HEY2
for.	GTA CCA TCC AGC AGT GCA TC	60
rev.	AGA GAA TTC AGT CAG GGC ATT T
HES1
for.	AGT GAA GCA CCT CCG GAA C	60
rev.	CGT TCA TGC ACT CGC TGA
VEGF
for.	GAA GTG GTG AAG TTC ATG GAT GT	60
rev.	TGG AAG ATG TCC ACC AGG GTC
GAPDH
for.	AGC CAC ATC GCT CAG ACA	58
rev.	GCC CAA TAC GAC CAA ATC C

Abbreviations: for., forward; rev., reverse.