Skip to main content
. Author manuscript; available in PMC: 2013 Sep 20.
Published in final edited form as: Mol Reprod Dev. 2009 Dec;76(12):1188–1199. doi: 10.1002/mrd.21092

Figure 2. Presence of Izumo 3 and 4 on sperm.

Figure 2

A: Acrosome-intact sperm were extracted with PFO and the proteins were separated by SDS-PAGE and transferred to a PVDF membrane. The membranes were probed with immune (I) or pre-immune (PI) sera specific for each protein at a 1:1000 dilution. B: Effect of treatment with alkaline phosphatase. Sperm extracts were treated (+) or not (−) with alkaline phosphatase (AP) and the effect on the MW of Izumo 1 and Izumo 4 was evaluated by SDS-PAGE and Western blot. C: Reactivity of the Izumo 1 forms with antibodies 2417 (against the extracellular domain) or 3149 (against a cytoplasmic region).