Skip to main content
. Author manuscript; available in PMC: 2013 Sep 20.
Published in final edited form as: Mol Reprod Dev. 2009 Dec;76(12):1188–1199. doi: 10.1002/mrd.21092

Figure 3. Localization of Izumo members on sperm and fate after the AR.

Figure 3

A: Freshly isolated sperm were disrupted by sonication and head and tail fractions were isolated by velocity sedimentation on sucrose gradients. 2 × 106 isolated sperm heads or tails were loaded per lane and the presence of the Izumo members in the samples was analyzed by Western blot. B: Proteins from acrosome-intact (AI) and acrosome-reacted (AR) sperm were separated by SDS-PAGE and the presence of the different Izumo members was analyzed by Western blot. The percentage of acrosome –reacted cells was 84%.