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. Author manuscript; available in PMC: 2013 Sep 20.
Published in final edited form as: Mol Reprod Dev. 2009 Dec;76(12):1188–1199. doi: 10.1002/mrd.21092

Figure 4. Immunofluorescence localization of Izumo 1 and Izumo 3 on sperm.

Figure 4

Acrosome-intact or acrosome-reacted sperm were fixed, permeabilized and incubated with antibodies 2417 or 3149 against Izumo 1 or antibodies against Izumo 3. I: immune sera, PI: pre-immune sera. The percentage of acrosome reaction was 77–81%.