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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Biochim Biophys Acta. 2013 Jun 15;1834(10):2045–2056. doi: 10.1016/j.bbapap.2013.06.003

Figure 8. Thermodynamic characterization of the interaction between purified CB2 and an anti CB2 monoclonal antibody by SPR.

Figure 8

The normalized binding response of NAA-1 antibody to CB2–coated surfaces as a function of antibody concentration is shown. For normalization, the response obtained from equilibrium titration experiments (Material and methods, and figure S4) at the end of each antibody injection (0-450 nM) was divided by the corresponding surface density RU-value of captured CB2. Steady state affinity analysis was performed using GraphPad Prism software. The value for KD (25 °C) of 2.2 ± 0.2 nM was obtained by non-linear regression analysis considering a 1:1 binding model.