. Author manuscript; available in PMC: 2014 Oct 1.

Published in final edited form as: Biochim Biophys Acta. 2013 Jun 15;1834(10):2045–2056. doi: 10.1016/j.bbapap.2013.06.003

Table 2. Binding of monoclonal anti- CB₂ antibodyNAA-1 to surface-immobilized CB₂.

The calculation of the fraction of CB₂ that binds NAA-1 is explained in appendix B.

Running Buffer	Source of protein	Protein immobilized	Treatment after immobilization	Running Buffer	Antibody binding (%)
TBS-T	Homogeneous preparation	CB2	none	TBS-T	64%
TBS-T	Cell Iyzate	Fusion	none	TBS-T	20%
C-SPR	Cell Iyzate	Fusion	none	C-SPR	2%
C-SPR	Cell Iyzate	Fusion	TEV protease cleavage	C-SPR	60%

Table 2. Binding of monoclonal anti- CB2 antibodyNAA-1 to surface-immobilized CB2.