Fig. 1.

Ectopic expression of αB-crystallin increases E-selectin levels in HUVEC. a Representative Western Blot for αB-crystallin in HUVEC transduced with a lentiviral vector coding for full-length human αB-crystallin (pgk:cryab) or an empty control vector (pgk:ev). Actin served as loading control. b Representative FACS plots of E-selectin staining on HUVEC transduced with pgk:cryab and pgk:ev after treatment with TNF-α for 24 h. c Quantification of surface expression of E-selectin in HUVEC transduced with pgk:cryab (white bars) or empty vector controls (black bars). Cells were treated with TNF-α for 5 h (left panel) or 24 h (right panel) d HUVEC transduced with pgk:cryab (white bars) or empty vector controls (black bars) were stimulated for 3, 6, 12 and 24 h with TNF-α and expression of E-selectin relative to hprt was determined by qPCR (Bars represent mean ± SD fold expression compared to pgk:ev after 3 h of TNF-α (normalized data from 3 independent experiments), * = p < 0.05). e HUVEC transduced with pgk:cryab (white bars) or empty vector controls (black bars) were grown to confluency and stimulated with TNF-α for 24 h before co-incubation with Jurkat cells for 15 min. Firmly adherent cells were microscopically quantified. (Bars represent mean ± SD (normalized data from 3 individual independent), * = p < 0.05)