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. Author manuscript; available in PMC: 2014 Mar 4.
Published in final edited form as: Neuron. 2013 Sep 4;79(5):903–916. doi: 10.1016/j.neuron.2013.06.035

Figure 3. The EBAX-1-Containing CRL is Important for AVM Guidance.

Figure 3

(A) Illustration of EBAX-1 wild-type and mutant constructs. Dashed lines indicate deleted regions in EBAX-1 ΔBox and ΔSWIM mutants. Asterisks mark the BC-box and Cul2-Box with amino acid substitutions in EBAX-1 M1 and M2 mutants, respectively.

(B-C) Quantitative analysis of AVM guidance defects rescued by the EBAX-1 WT, ΔBox, M1, M2, or ΔSWIM mutant driven by the ebax-1 promoter in unc-6 (B) or unc-40 (C) mutant animals. Data combined from 2-4 transgenic lines of each EBAX-1 construct are shown. Data represent means ± SEM. ***p<0.001 and ###p<0.001 relative to indicated controls; ns, not significant; one-way ANOVA.

(D) The effect of touch neuron-specific cul-2 RNAi on AVM guidance in the unc-6(ev400) mutants. cul-2 dsRNAi animals expressed both Pmec-7-driven cul-2 sense and antisense RNA strands. cul-2 sense control animals only expressed the sense RNA strand. Two independent RNAi sequences (dsRNAi #1 and #2) were used. Data represent means ± SEM. ***p<0.001; one-way ANOVA.