(A) Interactions between DAF-21 and the EBAX-1 WT or truncated mutants in Y2H assays. + and ++, intermediate and strong interaction; –, no interaction.
(B) Genetic effects of daf-21 and ebax-1 mutants on AVM guidance.
(C) Rescue analyses of daf-21 and ebax-1 transgenes in double and triple mutants. EBAX-1 (+) and DAF-21 (+), overexpression of Pebax-1::EBAX-1 WT and Pdaf-21::DAF-21 WT in indicated mutant backgrounds. Data in (B) and (C) represent means ± SEM. *p<0.05, **p<0.01, and ***p<0.001 relative to the unc-6 single mutant; #p<0.05, ##p<0.01, and ###p<0.001 relative to indicated controls; one-way ANOVA.