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. Author manuscript; available in PMC: 2014 Jul 30.
Published in final edited form as: Biochemistry. 2013 Jul 18;52(30):5103–5116. doi: 10.1021/bi4005914

FIGURE 6.

FIGURE 6

The linear fluorescent tubulin-like structure (TLS) found in well-differentiated MDCK cells expressing SLC13A3_15 persisted after a 44-minute hypotonic shock. (Left) Images of XY midsection and merged XY and YZ sections in Hanks’ balanced salt solution with 0.5% FBS. (Right) Images of XY midsection and merged XY and YZ sections of cells treated with 25% osmolarity hypotonic shock at 37° C incubation for 44-minute. Hypotonic condition was created by adding 1.5 ml sterile water to the 0.5 ml Hanks’ balanced salt solution with 0.5% FBS in the chamber. Arrows: the location of cells that became deformed during the shock.