Figure 5. Altered phenotype of NIK KO Tregs depends on NIK KO stromal cells.

Single cell suspensions of nucleated splenocytes from intact (A and B), single BM chimeric (C and D), or mixed BM chimeric (E and F) mice were stained with the indicated fluorescently labeled antibodies. A, C, and E, Quantitation of percent CD62L^lo among Foxp3⁺ NIK KO or WT cells. B, D, and F, Quantitation of mean fluorescence intensity of CD44 and CTLA4 on Foxp3⁺ NIK KO or WT cells. Data represent mean ± SEM of n=3-4 mice per group in one representative experiment of two (A, B, E, F) or three (C, D).