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. 2013 Sep 20;8(9):e75833. doi: 10.1371/journal.pone.0075833

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© 2013 Czeisler et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Endothelial cells stained for alpha tubulin (red) and total VEGFR2 (green). (B) Inset from panel A shows cell in more detail. Two insets (B1) and (B2) from panel B separate channels in red and green to more clearly show the distinct pools of VEGFR2 present in endothelial cells. This includes a pool of VEGFR2 on the cell surface (arrowhead), a granular cytoplasmic VEGFR2 pool (black arrow) and a pool of VEGFR2 arranged in fibrous arrays (white arrow). (C) Endothelial cells stained for microfilament beta actin (red) and total VEGFR2 (green). (D) Inset from panel C shows cell in more detail. The insets (D1) and (D2) from panel D separate channels into red and green to demonstrate more clearly distinct pools of VEGFR2 present in endothelial cells. This includes a pool of VEGFR2 on the cell surface (arrowhead, green), a granular cytoplasmic VEGFR2 pool (black arrow) and a pool of VEGFR2 arranged in fibrous arrays (white arrow). (E) Cell stained for intermediate filament Vimentin (red) and total VEGFR2 (green). (F) Inset from panel E shows cell in more detail. Insets (F1) and (F2) taken from panel F separate channels in green and red to show more clearly the distinct pools of VEGFR2 present in endothelial cells. This includes a granular cytoplasmic VEGFR2 pool (black arrow) and a pool of VEGFR2 arranged in fibrous arrays (white arrow). All displayed images are single focal planes.