Table 1. N-terminal sequences of proDer p 3 zymogens obtained after purification at 4°C.
| Proteins | Expected and observed N-terminal sequences | Approximate (%) |
| proDer p 3 | NPILPASPQAT IVGGEKALAG … | |
| 4°C | EFNPILPASPQATIVGGEKALAG | 100 |
| P2A proDer p 3 | NAILPASPQAT IVGGEKALAG … | |
| 4°C | EFNAILPASPQATIVGGEKALAG | 100 |
| P5A proDer p 3 | NPILAASPQAT IVGGEKALAG … | |
| 4°C | EFNPILAASPQATIVGGEKALAG | 100 |
| P8A proDer p 3 | NPILPASAQAT IVGGEKALAG … | |
| 4°C | EFNPILPASAQATIVGGEKALAG | 100 |
| P-A proDer p 3 | NAILAASAQAT IVGGEKALAG … | |
| 4°C | EFNAILAASAQATIVGGEKALAG | 8 |
| ILAASAQATIVGGEKALAG | 5 | |
| ASAQATIVGGEKALAG | 9 | |
| SAQATIVGGEKALAG | 42 | |
| AQATIVGGEKALAG | 16 | |
| ATIVGGEKALAG | 14 | |
| ALAGE | 6 | |
| Δ1–2 proDer p 3 | ILPASPQAT IVGGEKALAG … | |
| 4°C | EFILPASPQATIVGGEKALAG | 88 |
| SPQATIVGGEKALAG | 3 | |
| Not identified | 9 | |
| Δ1–5 proDer p 3 | ASPQAT IVGGEKALAG … | |
| 4°C | EFASPQATIVGGEKALAG | 88 |
| ATIVGGEKALAG | 8 | |
| Not identified | 4 | |
| Δ1–8 proDer p 3 | QAT IVGGEKALAG … | |
| 4°C | EFQATIVGGEKALAG | 24 |
| EAEFQATIVGGEKALAG | 74 | |
| Not identified | 2 | |
| Δ1–11 proDer p 3 | IVGGEKALAG… | |
| 4°C | EAEFIVGGEKALAG | 94 |
| EAEAEFIVGGEKALAG | 6 |
All proteins contain the N9Q mutation, which abolishes N-glycosylation of the propeptide. The mature Der p 3 sequence is shown in italics. The EF N-terminal extension resulted from cloning.