Figure 2. Quiescence bout lengths in egl-30 mutants.

A Frequency distribution of quiescence bout durations for wild type and egl-30 mutants calculated as the fraction of lethargus length (or a time window of 2h during wake-like behavior). A bout was defined as immobility lasting 5s or longer. B Quantification of total immobility ≥ 5s calculated as a fraction of lethargus length (or a time window of 2h during wake-like behavior). C Cumulative immobility ≥ 5s integrated over the entire lethargus phase (or a time window of the same length during wake-like behavior). D Mean lethargus duration in hours as defined by the absence of pharyngeal pumping. E Mean development time in hours from 3-fold embryo until L2 larvae in wild type and egl-30 mutants. Error bar represent SEM. A paired sample Wilcoxon test was used for statistical testing of wake-like versus sleep-like behavior. T-test was used for comparing different genotypes.