Figure 4. Reduced muscle relaxation in egl-30 mutants during sleep-like behavior.

A False color fluorescence images of wild type and egl-30 mutants expressing GCaMP3.35 in body wall muscle during the wake-like state (top) and during the sleep-like state (bottom). Bright signals reflect muscle activity. The angle change is displayed for each example worm. B Mean angle change in wild type and egl-30 mutants during wake-like and sleep-like state. C Reduction of muscle calcium signals in wild type and egl-30 mutants. Note the reduction of angle change and muscle calcium during sleep-like behavior in wild type. Body curvature and muscle calcium are much less reduced in egl-30 mutants. Error bars represent SEM. For each strain ≥ 10 animals were tested (two-sample t-test for comparisons between different genotypes, paired sample Wilcoxon test for comparison of wake-like and sleep-like behavior, *** denotes statistical significance at p<0.001)..