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. 2013 Sep 20;8(9):e75124. doi: 10.1371/journal.pone.0075124

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© 2013 Nakano et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Alignment of deduced amino acid sequence of DS1 and its ortholog in N. tabacum (NtPAP), phosphatidic acid phosphatase 2-like proteins from Vitis vinifera (VvPAPLP), Thellungiella halophila (ThPAP2LP), Arabidopsis thaliana (AtPAP2LP), and Oryza sativa (OsPAP2LP). Dots show identical amino acids, bars show amino acids that are not present in sequences. Boxes show conserved PAP catalytic motifs I, II, and III essential for PAP activity. Putative transmembrane motifs are shown in italic with underline. (B) Phylogenic tree of lipid phosphatases in plants. Gray boxes show DS1 and its ortholog in N. tabacum. The scale bar represents 0.1 JTT distance matrix units.