Table 1. Primers used for amplification and sequencing of the entire H gene.
Primera | Sequence(5′-3′orientation) | Positionsb | Ampliconc |
MHs | GTGCAAGATCATCCACAATGTCACC | 7254-7278 | 1,919 bp |
MHas | GTATGCCTGATGTCTGGGTGA | 9172-9152 | |
H1s | GTGCAAGATCATCCACAATGTCACC | 7254-7278 | Seq |
H2as | GTCAGAGATGAATTTCAC | 7630-7613 | Seq |
H3s | TTGGTGAACTCAACTCTACTG | 7766-7786 | Seq |
H4as | GGA ACTGAGTTTGACATCAC | 7811-7792 | Seq |
H5as | GTATGCCTGATGTCTGGGTGA | 9172-9152 | Seq |
: s, sense orientation; as, antisense orientation.
: the primers' nucleotide locations and range on the basis of the Measles prototype strain: Edmonston complete genome (GenBank ID: AF266288).
: Length of the PCR product or use of the primer for sequencing (Seq).