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. 2013 Sep 20;8(9):e75002. doi: 10.1371/journal.pone.0075002

Figure 5. Expression of oxt, avp and trh in otpa and otpb single and double mutant larvae.

Figure 5

Whole-mount in situ hybridization reveals loss of oxt, avp and trh expression in the preoptic region (arrowhead) of otpa;otpb double mutant larvae at 3 dpf. We detected oxt-expressing cells at ectopic locations within the diencephalon in otpa mutants (B2, asterisk). A reduction of trh-expressing cells in the preoptic region in otpa single mutants is detectable, (J2, arrowhead). Dorsal view, anterior at left. Scale bar is 50 µm. Abbreviations: H, hypothalamus; PO, preoptic region.