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. 2013 Sep 20;8(9):e74843. doi: 10.1371/journal.pone.0074843

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© 2013 Yehuda-Shnaidman et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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HCT116 cells were treated for 24 hours with DMEM (control), CM collected from visceral AT of non-obese subjects (n=4) or obese subjects (n=10) and analyzed for their OCR (A) and ECAR (B) levels by using the XF24 Analyzer. *, P< 0.05 vs. non-obese or control (Student’s t-test). (C) Maximal OCR levels following FCCP (0.4 µM) were measured using the XF24 Analyzer. Control (n=9), non-obese (n=4), obese (n=10). *, P< 0.05 vs. non-obese sample (Mann Whitney test). Results were normalized to protein concentration and expressed as percentage of control.