Figure 7. Leptin involvement in mediating obesity-reduced OCR.
HCT116 cells were treated for 24 hours with CM collected from visceral AT of non-obese or obese subjects, with or without leptin antagonist (1 ng/ml). OCR levels were measured using the XF24 Analyzer. Non-obese (n=4), obese (n=7), non-obese antagonist (LepA non-obese, n=3), obese antagonist (LepA Obese, n=6). *, P< 0.05 vs. samples from obese (Student’s t-test). Results were normalized to protein concentration and expressed as percentage of control.