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(A) SDS-PAGE of purified MCR, MCR-N, and MCR-C proteins. (B) In vitro analysis of malonyl-CoA reductase activity. Enzyme activities were assayed as described in Materials and Methods. The reaction products of MCR, MCR-N, MCR-C, and MCR-N/MCR-C were analyzed using LC-MS. Representative results from duplicated independent experiments are shown, TIC, total ion chromatogram. MCA, malonyl-CoA. MSA, malonate semialdehyde.
