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(A) The location of amino acid changed in the MCR-N and MCR-C proteins that were tested. (B) In vitro analysis of malonyl-CoA reductase activity. Wild-type and mutated proteins were tested and the products were identified by LC-MS. In MCR-N reactions, wild-type MCR-C protein was used to convert malonyl-CoA into malonate semialdehyde, which is the substrate of MCR-N.
