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. 2013 Sep 20;8(9):e75084. doi: 10.1371/journal.pone.0075084

Figure 1. The B7 cytoplasmic domain can enhance the surface expression of a single-chain antibody.

Figure 1

a) scFv-B7-38 is composed of a signal peptide (SP), a HA epitope tag, a single-chain antibody (scFv) and the B7 transmembrane domain (TM) and 38 amino acid cytoplasmic domain (cyt). The terminal 33 amino acids are deleted in scFv-B7-5. b) 3T3 fibroblasts transiently transfected with plasmids expressing scFv-B7-38 or scFv-B7-5 were immunofluorescence stained for the HA tag present in the scFv and analyzed on a flow cytometer. c) scFv-PDGFR is identical to scFv-B7-38 except that the transmembrane and truncated cytoplasmic domain is derived from the platelet derived growth factor. The B7 cytoplasmic domain was appended to the C-terminus in scFv-PDGFR-B7. d) 3T3 fibroblasts transiently transfected with plasmids encoding scFv-PDGFR or scFv-PDGFR-B7 were immunofluorescence stained for the HA epitope in chimeric proteins on the surface of cells and analyzed on a flow cytometer.