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. 2013 Sep 20;8(9):e75084. doi: 10.1371/journal.pone.0075084

Figure 6. Transport of B7 chimeric proteins depends on Sar1 and Rab1 GTPases.

Figure 6

3T3 cells that stably expressed scFv-B7-38 were transiently transfected with eGFP-Sar1WT, mCherry-Sar1[T39N], eGFP-Sar1[H79G], eGFP-Rab1WT or mCherry-Rab1DN plasmids. Forty-eight hours later, the cells were immunofluorescence stained with anti-HA antibody to detect the levels of HA-tagged scFv-B7-38 on the surface of the cells. Results show the mean surface immunofluorescence levels of scFv-B7-38 on live cells that were successfully transfected and expressed eGFP or mCherry (expression positive cells, filled bars) compared to live cells that were not transfected and thus did not express eGFP or mCherry (expression negative cells, open bars).