Figure 10. The B7 cytoplasmic tail does not appear to possess a linear ER export motif.

a) The intracellular transport, as measured by the ratio of intracellular Golgi-glycosylated AFP to total intracellular AFP, of chimeric proteins with mutations in putative ER export motifs (underlined) or a deletion of amino acids 6 to 20 (AFP-B7(Δ6-20)) are shown. b) Alanine scanning analysis was performed over amino acids 20-38 in the B7 cytoplasmic domain. The intracellular transport rate, quantified as the percentage of intracellular Golgi-glycosylated AFP relative to total intracellular AFP, is shown.