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. 2013 Sep 20;8(9):e75084. doi: 10.1371/journal.pone.0075084

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© 2013 Lin et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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a, c) The frequency of individual amino acids in the extracellular (open bars) and cytoplasmic (solid bars) domains of 911 human (a) or 733 mouse (c) type I transmembrane proteins is shown. Significant differences in mean amino acid frequencies between extracellular and cytoplasmic domains are indicated: n.s., not significant; *, p ≤ 0.05; **, p ≤ 0.001; ***, p ≤ 0.0001. b, d) The relative preference of individual amino acids for the cytoplasmic domain relative to the extracellular domain of 911 human (b) or 733 mouse (d) type I transmembrane proteins was calculated as described in Materials and Methods. Positive values indicate a preference for the cytoplasmic domain.