Figure 13. Charged amino acids in the B7 cytoplasmic tail are not responsible for effective intracellular transport.
a) Acidic or both acidic and basic amino acids were replaced with glycine residues in AFP-B7-NE and AFP-B7-NC, respectively, whereas all amino acids except the charged amino acids were altered in AFP-B7-CS. The expression of chimeric proteins on the surface of transiently-transfected 3T3 cells was determined by flow cytometry. b) The percentage of intracellular Golgi-glycosylated AFP chimeras in relation to total intracellular AFP chimeric protein in 35S-methionine labeled cells at the indicated chase times is shown.