Figure 15. Correlation between predicted secondary structure of cytoplasmic tails and intracellular transport rate.

Relative cytoplasmic tail structure (degree of random structure) was calculated by dividing the predicted percentage of random coil structure by the sum of the predicted percentage of helical and extended strand structures in the cytoplasmic tails of AFP chimeric proteins as calculated by the secondary structure prediction program SSPro 2.01. A large value of this metric corresponds to a predicted lack of ordered structure. The relative transport rates of AFP chimeric proteins (transport rate) were estimated by calculating the time required for 50% of the chimeric protein to attain the Golgi glycosylated form (complex carbohydrate) from the pulse-chase experiments. The best fit linear regression line for the data is also shown (r² = 0.795).