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. Author manuscript; available in PMC: 2013 Sep 21.
Published in final edited form as: Hepatology. 2012 Mar 1;55(4):1193–1203. doi: 10.1002/hep.24790

Fig. 2.

Fig. 2

Generation of hepatocyte-like cells from human iPSCs. (A) Images showing the sequential morphological change from human iPSCs to hepatocyte-like cells. (Panel i) Morphology at the preinduction stage of human iPS cells; Panel ii shows the morphological changes that occur to give a spiky shape after culture in endodermal induction medium. (Panel iii) At day 7 after hepatic lineage commitment medium induction, the cell morphology has become polygonal in shape. (Panel iv) The morphology of the mature hepatocyte-like cells. (B) Immunocytochemisty showing that the expression of the definitive endoderm marker SOX17 at day 4 and (C) that the cells are positive for AFP and albumin at day 12 after the start of the differentiation procedure. Scale bars, 100 μm. (Original magnification, ×100). DAPI, 4′,6-diamidino-2-phenylindole.