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. Author manuscript; available in PMC: 2013 Sep 21.
Published in final edited form as: Hepatology. 2012 Mar 1;55(4):1193–1203. doi: 10.1002/hep.24790

Fig. 3.

Fig. 3

HGF plays an important role in endoderm formation during the hepatogenic differentiation of human iPSCs. (A) Immunocytochemical analysis of cells treated with endodermal induction medium with HGF or without HGF shows efficient production of the endoderm marker Foxa2 only in medium with HGF (C) Immunocytochemistry showing the expression of the colocalized definitive endoderm markers SOX17 and Foxa2 at day 5; the upper panel shows endodermal induction medium with HGF and the lower panel shows endodermal induction medium without HGF (Original magnification, ×100). (B,D) Quantitative analysis confirmed that there was a significant change between the cells grown in HGF+ and HGF− medium. Scale bars, 100 μm. (t test, n = 3). DAPI, 4′,6-diamidino-2-phenylindole.