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. Author manuscript; available in PMC: 2013 Sep 21.
Published in final edited form as: Hepatology. 2012 Mar 1;55(4):1193–1203. doi: 10.1002/hep.24790

Fig. 5.

Fig. 5

Functional analysis of the hepatocyte-like cells derived from iPSCs. (A) After 12 days induction, the human iPSC-derived hepatocytes exhibited cytochrome P450 isozyme activity similar to primary human hepatocytes (n = 3) and (B) they also secreted urea. (C) Immunofluorescence staining for LDL uptake in iPSC-derived hepatocytes. Glycogen storage was examined by PAS staining (D, panel i), which begins to show glycogen storage at differentiation day 12. (Panel ii) Glycogen stored in hepatic cells can be digested by diastase treatment and is then present as negative PAS staining. (Panels iii and iv) The primary human hepatocytes were used as a positive control. (Original magnification, ×100).