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Hepatogenic Differentiation Condition
| Induction period | Medium Contents | |
|---|---|---|
| Preinduction | Day −4~Day 0 | CM (MEF-conditioned medium) |
| Endodermal induction | Day 1~Day 3 | RPMI/B27, activin A (100 ng/mL), Wnt 3a (50 ng/mL), HGF (10 ng/mL) |
| Hepatic lineage commitment | Day 4~Day 7 | KO/DMEM, L-glutamine (1 mM), nonessential amino acids (1%) 2-Mercaptoethanol (0.1 mM), dimethyl sulfoxide (1%) |
| Maturation step | Day 8~Day 12 | IMDM, oncostatin M (20 ng/mL), dexamethasone (0.5 μM), ITS premix (50 mg/mL) |
