| HG1 |
5′-GGGGTTTTAATATTGAGAACGGTAATGTTTGTGCTGG-3′ |
| HG2 |
5′-GGGGTTTTGGATCCTATAAAATCCTCTTTGACTTTT-3′ |
| HG3 |
5′-GGGGTTTTCATATGGAGAACGGTAATGTTTGTGCTGG-3′ |
| HG4 |
5′-GGGGTTTTGAATTCTATAAAATCCTCTTTGACTTTT-3′ |
| HG50 |
5′-GGGGTTTTGAATTCTCTAGAGGATCCGTCGACCTGCAGATGAAATCTAACAATGCGCTCATCG-3′ |
| HG52 |
5′-GGGGTTTTAAGCTTTTATTAGTGATGGTGATGGTGATGGTGATGGTGATGCCCGGGGGTCGAGGTGGCCCGGCTCC ATGC-3′ |
| HG53 |
5′-GGGTTTGCATATGGAGAACGGTAATGTTTGTGC-3′ |
| HG54 |
5′-GGGTTTGGAAGCGGAAGAGCGGGTTATTGTCTCATGAGCGG-3′ |
| HG55 |
5′-GGGTTTTGAATTCATGGCTAGCATGACTGGTGGACAGCAAATGGGAGGAGCGGCCGCGAAATCTAACAATGCGCT CATCGTCATCC-3′ |
| HG56 |
5′ -GGGGTTTTAAGCTTTTATTAATGGTGATGGTGATGGTGATGGTGATGGTGGGCAGCGCGGCCGCCGGTCGAGGTGG CCCGGCTCCATGCACC-3′ |
| pHG2 |
cea promoter amplified from pGE124 |
| pHG4 |
tet gene cloned from pKRP12 and inserted into pHG2 |
| pHG5 |
cea promoter, I0×His tagged tet gene, rmBTlT2 fragment of pHG4 cloned into Ndel and Sapl sites of pUCI8 |
| pHG6 |
N-terminal T7 antigen tag and 5′ and 3′ LIC sites inserted into EcoRI-HindIII fragment of pHG5 |
| pHG7 |
pHG4 promoter, T7, His, and LIC cloning site in pUCI8 created by NotI digest of pHG6 and recircularizing the larger fragment (i.e. without tet)
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